ERBB4 (N-term) Peptide
产品名称: ERBB4 (N-term) Peptide
英文名称: ERBB4 (N-term) Peptide
产品编号: P2655
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ERBB4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ERBB4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment.
Cheng QC, Tikhomirov O, Zhou W, Carpenter G.J Biol Chem. 2003 Oct 3;278(40):38421-7. Epub 2003 Jul 16.
- 2.
- WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus.
Komuro A, Nagai M, Navin NE, Sudol M.J Biol Chem. 2003 Aug 29;278(35):33334-41. Epub 2003 Jun 13.
- 3.
- A constitutively active ErbB4 mutant inhibits drug-resistant colony formation by the DU-145 and PC-3 human prostate tumor cell lines.
Williams EE, Trout LJ, Gallo RM, Pitfield SE, Bryant I, Penington DJ, Riese DJ 2nd.Cancer Lett. 2003 Mar 20;192(1):67-74.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of ERBB4 polyclonal antibody (Cat # PAB3038) in HL-60 cell lysate. ERBB4 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ERBB4 polyclonal antibody (Cat # PAB3038) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 2066
- Protein Accession#:
- Q15303
- Gene Name:
- ERBB4
- Gene Alias:
- HER4,MGC138404,p180erbB4
- Gene Description:
- v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
- Omim ID:
- 600543
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq
- Other Designations:
- avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4,receptor tyrosine-protein kinase erbB-4,tyrosine kinase-type cell surface receptor HER4,v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4,v-erb-a erythroblastic leukemia v
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